Chromatography results can be compared with known samples and Rf values are used to to compare the substances. If the compounds are colourless it is necessary to use locating agents.
To perform a paper chromatography
you need a chromatography paper.
- Draw the starting line using a pencil. (Pen ink can contain pigments which can interfere with the results)
- Place a dot of the mixture you want to separate. (If you analyse more than one sample at a time load they with a suitable distance from each other)
- Dip the chromatography paper inside the solvent so that it touches the paper but below the starting line.
- Keep the setup inside a chromatography jar (otherwise the solvent can evaporate)
- Let the chromatography run for some time. when the solvent reaches the end of the paper take out the paper and mark the ending line
- Calculate the Rf values using the following equation
Rf=Distance traveled by the solute / Distance traveled by the solvent
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calculatr Rf value
chromatography
chromatography paper
GCSE Chemistry
how to perform a paper chromatography
locating agents
paper chromatography
rf value
